Coding
GroEL

Part:BBa_K205000:Design

Designed by: Michael James George Milevskiy   Group: iGEM09_UQ-Australia   (2009-09-08)

GroEL is a chaperone protein that prevents polypeptide misfolding.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 251
    Illegal PstI site found at 377
    Illegal PstI site found at 547
    Illegal PstI site found at 865
    Illegal PstI site found at 1093
    Illegal PstI site found at 1510
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 251
    Illegal PstI site found at 377
    Illegal PstI site found at 547
    Illegal PstI site found at 865
    Illegal PstI site found at 1093
    Illegal PstI site found at 1510
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 251
    Illegal PstI site found at 377
    Illegal PstI site found at 547
    Illegal PstI site found at 865
    Illegal PstI site found at 1093
    Illegal PstI site found at 1510
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 251
    Illegal PstI site found at 377
    Illegal PstI site found at 547
    Illegal PstI site found at 865
    Illegal PstI site found at 1093
    Illegal PstI site found at 1510
    Illegal AgeI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Gene was provided by Christos Kyratsous, we used mutagenesis to remove the internal restriction site, BamHI.

Source

Christos Kyratsous Columbia Univ, Coll Phys & Surg, Dept Microbiol, New York, NY 10032 USA

Ref: Kyratsous, CA, Silverstein, SJ, DeLong, CR, Panagiotidis, CA. (2009) "Chaperone-fusion expression plasmid vectors for improved solubility of recombinant proteins in Escherichia coli" GENE VOL: 440 Issue: 1-2 Pages: 9-15 Date: Jul 1 2009

References

GroEL: http://www.uniprot.org/uniprot/P0A6F5